Development of novel methods to regulate only papthologically activated osteoclasts: A novel Concept for the Osteoclast Activation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15677
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: KUKITA TOSHIO 九州大学, 歯学研究院, 教授 (70150464)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 破骨細胞 / 病的活性化 / 病的骨破壊 / 膜表面分子 / プロテオミクス / 酸感受性蛍光プローブ

Outline of Final Research Achievements

The purpose of this study is to search and find membrane molecules specific to the pathologically activated osteoclasts (PAOC), which acquired the highest bone destruction activity. As osteoclasts formed in the presence of IL-1β showed extremely high proton-secretion and bone resorbing activity, we have regarded these osteoclasts as PAOC. Data showed a marked suppression in the expression of molecules regulating terminal differentiation of normal osteoclasts and activation of integrinβ3. By use of the analysis of the cell surface proteins, we have detected the cell surface molecule specifically expressed in PAOC. Through this study, we could establish the experimental basis for the development of novel therapy targeting only PAOC.

Free Research Field

口腔解剖学