2016 Fiscal Year Final Research Report
A novel regulatory mechanism of NF-kB signaling
Project/Area Number |
15K15686
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
|
Research Institution | Kyushu Dental College |
Principal Investigator |
Eijiro Jimi 九州歯科大学, 歯学部, 教授 (40276598)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | NF-kB / 活性化機構 |
Outline of Final Research Achievements |
To investigate the physiological role of the serine residue at position 534 of the NF-κB, p65 subunit, we generated knock-in mice (S534A mice) in which the serine residue at position 534 was substituted with alanine. We established mouse embryonic fibroblasts (MEF) derived from wild-type and S534A mice. When we treated with TNFα, the nuclear translocation of p65 persisted for a longer time and enhanced transcriptional activity of NF-κB in S534A cells compared with wild-type cells. These results led us to, thought that there is phosphorylation-specific negative feedback mechanism of p65 S534. Therefore, we identified a novel molecule binding specifically to phosphorylation of p65 S534, and further revealed a new regulatory mechanism by NF-κB.
|
Free Research Field |
生化学、分子生物学
|