A novel regulatory mechanism of NF-kB signaling

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15686
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: Eijiro Jimi 九州歯科大学, 歯学部, 教授 (40276598)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: NF-kB / 活性化機構

Outline of Final Research Achievements

To investigate the physiological role of the serine residue at position 534 of the NF-κB, p65 subunit, we generated knock-in mice (S534A mice) in which the serine residue at position 534 was substituted with alanine. We established mouse embryonic fibroblasts (MEF) derived from wild-type and S534A mice. When we treated with TNFα, the nuclear translocation of p65 persisted for a longer time and enhanced transcriptional activity of NF-κB in S534A cells compared with wild-type cells. These results led us to, thought that there is phosphorylation-specific negative feedback mechanism of p65 S534. Therefore, we identified a novel molecule binding specifically to phosphorylation of p65 S534, and further revealed a new regulatory mechanism by NF-κB.

Free Research Field

生化学、分子生物学