2016 Fiscal Year Final Research Report
UVA-activated riboflavin inhibits demineralization of human dentin
| Project/Area Number |
15K15700
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MINOSHIMA Koji 大阪大学, 大学院工学研究科, 教授 (50174107)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 歯科保存学 / 根面う蝕 / コラーゲン / 紫外線 / リボフラビン / 象牙質 |
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| Outline of Final Research Achievements |
Flexural strength is the highest in the group of 0.1% riboflavin exposure and UVA irradiated with 1600 mW/cm2 for 10min, which was significantly higher than control group. Raman spectra showed that a C-C bond in a proline ring was amplified, and the ratio between C-N bond and N-H bond was changed by the riboflavin/UVA treatment. SDS-PAGE analysis suggested that the molecular weight of collagen was increased after the riboflavin/UVA treatment due to new collagen crosslinking.
In μCT analysis, the amount of mineral loss after demineralization in the RF/UVA group was significantly lesser than the control group. In SDS-PAGE analysis, the collagen after the collagenase degradation was less soluble in the riboflavin/UVA group than in the control group because of new collagen crosslinking promoted by a radical reaction.
Riboflavin/UVA treatment was effective in enhancing the acid and enzymatic resistance of human dentin by producing new collagen crosslinking.
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| Free Research Field |
医歯薬学
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