2016 Fiscal Year Final Research Report
The study on the pathogenic mechanisms of neuropathic pain using optical clearing method for brain
| Project/Area Number |
15K15727
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 神経障害性疼痛 / 三叉神経脊髄路核 / 可塑性 / グリア / 透明化 |
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| Outline of Final Research Achievements |
Although the pathogenic mechanisms of neuropathic pain remain to be elucidated so far, it has revealed recently that glial cells play a key role in the plastic change in the nociception. Therefore, the interaction between glial cells and neurons in the primary relay nuclei is important for the elucidation of the mechanisms of neuropathic pain. In this study, the optical clearing method "Scales" was applicated to the horizontal slices of rat medulla oblongata and immunohistochemical observation of the spinal trigeminal nucleus using immunofluorescence method was undertaken. In consequence, SN ratio of fluorescence image was insufficient and further trials of various conditions of sample preparation and/or staining conditions were thought to be essential.
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| Free Research Field |
歯科麻酔学
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