We investigate the essence of osseointegration and biointegration in the cell

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15741
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MIYAMOTO Youji 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (20200214)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: イオンプレーティング法 / マイクロアレイ解析 / マウス骨芽細胞様細胞 / ヒト骨髄間葉系幹細胞

Outline of Final Research Achievements

Human mesenchymal stem cell (h-MSC) and MC3T3-E1cells were cultured on the glass dishes which were coated by titanium, gold or stainless steel. And the change of the genes and micro RNA (miRNA) expressions by cell cultures on those different dishes were analyzed by the microarray assay. We identified 2 genes and 5 miRNA in h-MSC cultured on titan coated dishes more than twice expression as comparison with cell cultured on other dishes.
We also found the half or less expression of 2 genes and 2 miRNA decreased by cell culture on titan coated dishes. Similarly, in MC3T3-E1cells, we identified genes and miRNA related with cell attachment on titan.

Free Research Field

生体材料