3D analysis of the oral mucosa epithelium barrier function

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15747
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: KATO SHIN 慶應義塾大学, 医学部(信濃町), 助教 (80383719)
• Co-Investigator(Kenkyū-buntansha): 角田 和之 慶應義塾大学, 医学部(信濃町), 講師 (60265915)
• Co-Investigator(Renkei-kenkyūsha): SATO Hidekazu
• Research Collaborator: AMAGAI Masayuki ; KUBO Ryouji ; FUJITA KOHEI
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: タイトジャンクション / 口腔粘膜 / 三次元的観察

Outline of Final Research Achievements

On the basis of the reported cofocus microscope and observation method of the tight junction in the mouse auricle epithelium using the 3D construction software, I examined a condition such as one of reagent density so far at dyeing time to establish the observation method of the tight junction suitable for an oral mucosa epithelium more. As a result, observation of ZO-1 which was Claudin-1 which was the constitution protein of the tight junction of the mouse oral mucosa than a conventional method more clearly, Claudin-4 and lining protein was enabled by increasing primary dyeing time.

Free Research Field

口腔外科