Structural analysis of collagen-binding-proteins from Streptococcus mutans

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K15749
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Osaka Medical College
• Principal Investigator: Hirata Azumi 大阪医科大学, 医学部, 准教授 (40263587)
• Co-Investigator(Kenkyū-buntansha): 野村 良太 大阪大学, 歯学研究科, 准教授 (90437385) ; 高野 和文 京都府立大学, 生命環境科学研究科, 教授 (40346185)
• Research Collaborator: GOLDBERG Michel Université Paris Descartes, INSERM UMR-S 1124・Biomédicale des Saints Pères, Professeur Emerite
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: コラーゲン結合タンパク質 / Streptococcus mutans / 構造解析 / 機能解析

Outline of Final Research Achievements

Collagen-binding-proteins of Streptococcus mutans, Cnm/Cbm, have been reported to be as risk factors associated with bacteremia, cardiovascular diseases, and hemorrhagic stroke. In this study, the crystal structure of collagen-binding-domain of Cnm, Cnm30-325, was determined by molecular replacement methods, using the Cna31-344 crystal structure as a search model. The structure of collagen-binding-domain of Cbm, Cbm31-330, was modeled by SWISS-MODEL using the structure of Cnm30-325. The crystal structure of Cnm30-325 exhibits two distinct domains called N1 and N2. The structure of Cbm31-330 is very similar to the structure of Cnm30-325. N1, N2 and Linker form the hole and multiple sites in this area are related to bind to collagen, suggesting that it may be collagen-binding site.

Free Research Field

口腔解剖学

Academic Significance and Societal Importance of the Research Achievements

Cnm/Cbmにおいて、本研究により明らかとなったコラーゲンとの相互作用に関わる領域は、既知のコラーゲン結合タンパク質Cnaの領域と共通しており、この部位はコラーゲン結合タンパク質においてコラーゲンとの結合に関わる最も重要な部位であることが示唆された。