2017 Fiscal Year Final Research Report
Early closure of alveolar cleft by tissue regeneration using umbilical stem cells
| Project/Area Number |
15K15759
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Meikai University |
|---|
Principal Investigator |
Suda Naoto 明海大学, 歯学部, 教授 (90302885)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 口蓋裂 / 再生 / 骨 / 臍帯幹細胞 / 顎裂 |
|---|
| Outline of Final Research Achievements |
Human umbilical cells (UC-EZ) were derived from enzymatic digestion. Using magnetic-activated cell sorting (MACS), CD146-positive mesenchymal stem cells (US-MACS) were isolated. Two kinds of cells were positive for stem cell markers. UC-EZ were positive for Nanog and Oct3/4 but negative for Sox2. Two cells formed alizarin red-positive calcified matrix and oil red O positive lipid droplets. UC-MACS were positive for all stem cell markers and undifferentiated maintenance-related genes. UC-MACS showed high capacity to differentiate toward osteoblasts and adipocytes.
|
| Free Research Field |
歯科矯正学
|
