Synthesis of artificial genome consisting of only proteins used by early life

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Life / Health / Medical informatics
• Research Institution: The University of Tokyo
• Principal Investigator: Amikura Kazuaki 東京大学, 大学院新領域創成科学研究科, 助教 (60735918)
• Research Collaborator: Kiga Daisuke 早稲田大学, 先進理工学部・電気・情報生命工学科, 教授 (30376587)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 合成生物学 / 無細胞翻訳系 / リボソーム / tRNA / リポソーム / 構成的アプローチ

Outline of Final Research Achievements

This study is a research contributing to the development of basic technologies for artificial genome construction. In this study, we developed a system which exhibits desired function by cooperation of multiple kinds of proteins in cell-free translation system. In particular, we were focused on ribosome-related genes and other genes. The in vitro reconstitution system of ribosome using recombinant protein was able to construct a system with higher reconstitution efifciency of ribosome by using biosynthesis factor. In addition, new findings on the function of each factor in the ribosome biosynthesis process were presented.

Free Research Field

合成生物学