Enhancement of digestion and absorption function in small intestine by dietary components through transcriptional elongation factor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: HONMA Kazue 静岡県立大学, 食品栄養科学部, 助教 (80724765)
• Co-Investigator(Renkei-kenkyūsha): GODA Toshinao 静岡県立大学, 食品栄養科学部, 教授 (70195923) ; MOCHIZUKI Kazuki 山梨大学, 総合研究部, 教授 (80423838)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヒストン修飾 / フルクトース / 酪酸 / 小腸

Outline of Final Research Achievements

Dietary components can lead to epigenetic alterations, which affect gene expression in the cells. In this study, we focused on the transcriptional regulation of the genes related with nutrient metabolism through epigenetic alterations and investigated histone acetylations around the fructose- and/or short chain fatty acid-responsive genes. Re-feeding a diet containing sodium butyrate for 12 h after starvation in rats led to an increase in the mRNA levels of Si in the jejunum compared with the animals refed a control diet. We have also demonstrated that the acetylation level of histone H3 and the binding of CDK9, which phosphorylates RNA polymerase II, were enhanced in the rats refed a diet containing sodium butyrate at transcription regions of Si gene, which suggests a promotion of transcriptional elongation.

Free Research Field

分子栄養