Fabrication of a microfluidic device for circulating tumor cell sensing

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16320
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TABATA Miyuki 東京医科歯科大学, 生体材料工学研究所, テニュアトラック助教 (00636839)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: バイオセンサ / 血中循環腫瘍細胞

Outline of Final Research Achievements

In this research, we performed to fabricate a compact and portable device that detect circulating tumor cells (CTC) and analyze nucleic acids using ultra-sensitive biosensor based-on the principle of field effect transistors (FETs). Model breast cancer epithelial cells were immobilized on the sensor which the probe molecules were immobilized, and then electrical measurement was performed. Because cancer cells have a negative charge derived from sialic acids, we succeeded in electrically discriminating cancer cells and epithelial cells according to the difference in the amount of sialic acid expression on cell membrane. Furthermore, we tried to develop an early diagnosis device for cancer by analyzing the nucleic acid.

Free Research Field

バイオセンサ