Characterization of adipose tissue derived stromal vascular fraction cells with high angiogenic potential

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16329
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Nara Institute of Science and Technology (2017); National Institute of Advanced Industrial Science and Technology (2015-2016)
• Principal Investigator: TAKADA Hitomi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80641068)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 間葉系幹細胞 / 再生医療 / 1細胞解析

Outline of Final Research Achievements

Mesenchymal stem cells (MSCs) are promising cell source for regenerative medicine. However, the global picture of cell-type composition in MSCs has not been clarified. In this study, we used a newly developed high-throughput single-cell RNA-seq method, Quartz-Seq2, to analyze gene expression of freshly prepared stromal vascular fraction obtained from mouse adipose tissue. Dimensionality reduction and clustering of single-cell transcriptome data showed the separation of 11 clusters. Among them, we identified two types of CD34 positive MSCs, Efemp1+ MSCs and Col15a1+ MSCs. To investigate the difference between the two MSC clusters, we analyzed differentially expressed genes. Efemp1+ MSCs were characterized by the expression of growth factors, while Col15a1+ MSCs were characterized by the expression of genes that encodes extracellular matrix proteins. These data showed that the MSC population is divided into two clusters, suggesting that there is less heterogeneity of MSCs than expected.

Free Research Field

幹細胞生物学