2016 Fiscal Year Final Research Report
Development of a simultaneous detection system for nucleic acids using ratiometric NMR molecular probes
| Project/Area Number |
15K16560
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Wakayama University (2016)
Japan Advanced Institute of Science and Technology (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 同時一斉計測 / 核酸 / 19F NMR / 分子プローブ |
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| Outline of Final Research Achievements |
To understand molecular networks in life phenomenon, simultaneous and quantitative detection method for biomolecules is required. In this study, we tried to develop molecular probes that can detect various DNA on a 19F NMR spectra. Two different types of the probes, i.e. oligoDNA- and DNA binding small molecule-based probes, were synthesized and these abilities on DNA detection were evaluated. Results indicated that these probes can detect 4 different single-stranded DNAs and 4 different double-stranded DNAs simultaneously on a 19F NMR spectra.
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| Free Research Field |
生物有機化学
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