2016 Fiscal Year Final Research Report
Development of the molecular transfer based oligonucleotide sensing probe
Project/Area Number |
15K17876
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Analytical chemistry
|
Research Institution | Gifu University |
Principal Investigator |
SHIBATA Aya 岐阜大学, 工学部, 助教 (50462693)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | 遺伝子検出 / 芳香族求核置換反応 |
Outline of Final Research Achievements |
There is chemical reaction probe as genetic detection method in live cell. This strategy exploits the target strand as a template for two functionalized probes and provides a simple molecular mechanism for multiple turnover reactions. We tried development of the molecular transfer based oligonucleotide sensing probe which is triggered by a nucleophilic aromatic substitution (SNAr) reaction. We synthesized the molecular-transfer probe which combined methyl orange (MO). In using this probe, we confirmed that MO transfers under the target DNA existence.
|
Free Research Field |
分析化学
|