Development of the molecular transfer based oligonucleotide sensing probe

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K17876
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Gifu University
• Principal Investigator: SHIBATA Aya 岐阜大学, 工学部, 助教 (50462693)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 遺伝子検出 / 芳香族求核置換反応

Outline of Final Research Achievements

There is chemical reaction probe as genetic detection method in live cell. This strategy exploits the target strand as a template for two functionalized probes and provides a simple molecular mechanism for multiple turnover reactions.
We tried development of the molecular transfer based oligonucleotide sensing probe which is triggered by a nucleophilic aromatic substitution (SNAr) reaction. We synthesized the molecular-transfer probe which combined methyl orange (MO). In using this probe, we confirmed that MO transfers under the target DNA existence.

Free Research Field

分析化学