2016 Fiscal Year Final Research Report
Development of flow electrochemical immunoassay using carbon nanodots
| Project/Area Number |
15K17878
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 電気化学発光 / フローインジェクション分析 / マイクロ流路 / ジフェニルエーテル |
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| Outline of Final Research Achievements |
Carbon nanodots were synthesized from a mixing solution of citric acid and ethyrenediamine. We revealed the photophysical and electrochemical properties including electrogenerated Chemiluminescence. Moreover, carbon nanodots were chemically modified to an antigen, 3-phenoxybenzoic acid (3PBA), which is an metabolite of diphenylether harbicide in soil. The anti-3PBA antibody was immobilized on PDMS channel, and we performed immunoassay. In the detection part, we carried out electrochemical, fluorescence, and electrogenerated chemiluminescence detection, and found that fluoresce detection was the most sensitive method among them.
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| Free Research Field |
電気分析化学
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