2016 Fiscal Year Final Research Report
Design of protein binders based on the activation energy of interaction
Project/Area Number |
15K17882
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
NAGATOISHI SATORU 東京大学, 大学院工学系研究科(工学部), 助教 (30550248)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | 蛋白質 / 薬剤 / 熱力学 / 速度論 / 活性化エネルギー |
Outline of Final Research Achievements |
This study focused on the process of the interaction. Since it is assumed that a small compound forms some intermediate states with various amino acids and finally binds to the energy-stable site of protein, the process is possibly differ among proteins. Here, the binding kinetic analysis was carried out to elucidate the binding process of the compound to protein. In this study, complexed energy transition in the binding process is simplified as two state reaction and the state where a compound is in process is regarded as transition state. From the analysis of thermodynamic parameters in transition state, the transition state is examined at amino acid level and the relationship between transition state and binding process is discussed. This allows us to verify the possibility of obtaining an important indicator for drug design.
|
Free Research Field |
創薬物理化学
|