Development of highly elastic biomaterials based on resilin-like polypeptides

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K17913
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Polymer/Textile materials
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Tokuma Fukuoka 国立研究開発法人産業技術総合研究所, 機能化学研究部門, 主任研究員 (90415737)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: タンパク質 / 弾性繊維 / バイオベース材料

Outline of Final Research Achievements

Resilin is an elastic protein expected as a highly elastic biomaterial to replace synthetic rubbers. In this study, to obtain a novel highly elastic artificial polypeptide by genetic technology, several artificial genes encoding repetitive amino acid sequences of resilin were designed, synthesized, and transformed to Escherichia coli. Production conditions of objective polypeptides were investigated by cultivation of recombinant bacteria. The obtained polypeptides were readily isolated and purified by affinity chromatography as His-tag polypeptides.

Free Research Field

高分子化学