Microvoid cell manipulation and its application into porus-tissue engineering

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18004
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Nobuyuki 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (00724692)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞操作 / 物理化学 / 表面張力 / 細胞培養 / 組織工学

Outline of Final Research Achievements

In this research, we aimed to realize a high throughput cell manipulation method utilizing physico-chemical properties. During the research period, we developed a cell patterning technique using bubbles and interfacial properties, especially using surface tension as a key word.
When many bubbles with uniform size are generated in liquid, they can spontaneously be aligned in a hexagonal shape. By further pouring the liquid containing the cells into the aligned bubbles, the surface of the aligned bubbles became a kind of wall for cells, resulting in that hexagonal-patterned cell culture system was realized. As another candidate of "cell wall", we suggested that agar was quite useful material. In addition, by increasing the number of cells, we also performed the fabrication of thicker cellular tissue.

Free Research Field

機械工学