2016 Fiscal Year Final Research Report
Development of transmembrane protein array using virus-like particles
| Project/Area Number |
15K18274
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Shizuoka University |
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Principal Investigator |
Kato Tatsuya 静岡大学, 農学部, 准教授 (00397366)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | ウイルス様粒子 / 膜タンパク質 |
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| Outline of Final Research Achievements |
An antigen of Neospora caninum、SAG1 was displayed on the surface of Rous sarcoma virus-like particles (RSV VLPｓ), which were prepared in silskworm larvae. These RSV VLPｓdisplaying SAG1 were purifed by sucrose density gradient centrifugation and the displyed SAG1 was detected using epoxy group-modified glass slides.
In addition, core protein of hepatitis C virus (HCV) and its antibody were prepared in Escherichia coli and silkworm pupae and purifed by affinity chromatographies. Binding of the antibody to HCV core protein was detected using epoxy group-modified glass slides.
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| Free Research Field |
Biotechnology
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