Identification of T cells that exhibit cytotoxic activity against tumor based on the single-cell gene expression profiles and control their function

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Osaka University
• Principal Investigator: Morimoto Soyoko 大阪大学, 医学系研究科, 寄附講座助教 (10649023)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: single cell RT-PCR / WT1 specific CTLs / memory

Outline of Final Research Achievements

Multiple gene-expression analysis using single-cell real-time PCR showed that WT1 specific effector-memory CTL (WT1-EM CTL) population from cancer patients could be divided into two groups: the “activated” and “quiescent” states and WT1-EM CTL in good responder turn from “activated (Group: A)” to “quiescent (Group: B)” after WT1 peptide vaccination. We further show that TCR avidity could have played an essential role in differentiation of self-antigen-reactive T cells.

Free Research Field

癌免疫学