Establishent of two independent methods to analyze 5hmC at single base resolution

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18456
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Institute of Physical and Chemical Research (2016); Osaka University (2015)
• Principal Investigator: Takahashi Saori 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員 (80748856)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: DNAメチル化 / DNA脱メチル化 / ヒドロキシメチルシトシン / ヒストン修飾

Outline of Final Research Achievements

Cytosine methylation in genome contributes to gene silencing through changing chromatin state. At present, hydroxymethylcytosine (5hmC) produced from methylcytosine (5mC) by TET oxigenase is thought to be an possible intermediate for demethylation. Hence, a technique to analyze 5hmC at single base resolution in genome contributes to understanding of demethyltion processes. However, the reported techniques to analyze 5hmC harbor some disadvantages. In this period, I have successfully established two independent method to analyze 5hmC at single resolution, as I planned. One is enzymatical method and the other is chemical one. From my research, highly reproducible results are able to be obtained by these two easy methods. Thus, this project could contribute to the advance of functional understanding of 5hmC.

Free Research Field

エピゲノム