2017 Fiscal Year Final Research Report
Comprehensive analysis of genome imprinting regulation
| Project/Area Number |
15K18458
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | エピジェネティクス / ゲノムインプリンティング / enChIP / CRISPR/Cas9 / 幹細胞 |
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| Outline of Final Research Achievements |
Epigenetic gene regulation has critical roles for various physiological phenomena including development and diseases. Genomic imprinting is a typical epigenetic mechanism by which imprinted genes are expressed in a parent-of-origin specific manner. In this study, I searched for protein factors involved in imprinting regulation by combining enChIP, a new strategy developed from the CRISPR/Cas9 system, and special cell lines produced by nuclear transfer technology of fertilized egg. A lenti virus vector that expresses PA-tagged dCas9 for enChIP experiments was constructed and has been deposited to RIKEN BRC and Addgene. By using this construct, I identified proteins that bind to imprinting control regions in a DNA methylation dependent manner. It is suggested that one of these factors is possibly involved in a DNA methylation control mechanism.
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| Free Research Field |
エピジェネティクス、幹細胞学
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