2016 Fiscal Year Final Research Report
Identification of the RNA elements that determine semi-extractability of RNAs
| Project/Area Number |
15K18474
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | RNA抽出 / 核内構造体 / lncRNA / NEAT1 / RNAシークエンス / CRISPR/Cas9 |
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| Outline of Final Research Achievements |
NEAT1 architectural RNA (arcRNA) is an essential core of the nuclear body called paraspeckle. We have recently found that a subset of RNAs are hard to be extracted by the AGPC reagent. We termed this feature “semi-extractable”. Heating with shaking can make semi-extractable RNAs soluble. We investigated what RNA sequences and binding proteins generate this semi-extractable feature by using NEAT1 lncRNA as a model. Then we identified the middle domain of the NEAT1 is required for its semi-extractability and RNA binding proteins such as FUS, which binds to the middle domain of the NEAT1. By utilizing this semi-extractable feature in RNA-seq, we have identified the tens of semi-extractable RNAs in cell lines. Some of them form nuclear bodies like the paraspeckles, suggesting the utility of this method to identify new arcRNAs.
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| Free Research Field |
分子生物学、生化学、細胞生物学
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