2017 Fiscal Year Final Research Report
In vivo analysis of microRNA-mediated post-transcriptional regulation
| Project/Area Number |
15K18476
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 発生 / マイクロRNA / ゼブラフィッシュ |
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| Outline of Final Research Achievements |
Tnrc6 protein family had been considered as an essential factor in microRNA (miRNA)-mediated gene silencing based on previous researches using cultured cells. In this study, we established zebrafish genetic mutant of tnrc6a and tnrc6b and revealed that tnrc6 proteins are indeed essential for miRNA-mediated gene silencing during vertebrate development. We also discovered that codon composition determines mRNA stability independently of miRNA-mediated silencing. Furthermore, we successfully inhibited Dcp2 and Cnot7, two enzymes involved in mRNA decay, in zebrafish embryos. By performing RNA-seq analysis, we revealed the genome-wide requirement of the two enzymes during early embryogenesis.
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| Free Research Field |
分子生物学
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