2016 Fiscal Year Final Research Report
Regulatory mechanism for timely DARS2 activation
| Project/Area Number |
15K18479
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
MAYANAGI Kota 九州大学, 生体防御医学研究所 附属生体多階層システム研究センター, 助教
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | DNA複製 / 核様体蛋白質 / IHF / 細胞周期 |
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| Outline of Final Research Achievements |
Chromosomal DNA replication is rigidly regulated to initiate only once per cell cycle. In Escherichia coli, initiation regulation is sustained by multiple regulatory systems for the initiator DnaA and the unique replication origin oriC. A nucleoid protein IHF binds to oriC and other regulatory DNA factors datA and DARS2, and regulates DnaA activity and initiation timing. First, I found some candidates for IHF regulator through in vitro proteomic search. In addition, I revealed that DNA supercoiling plays a significant role in regulating datA-IHF binding and that chromosomal DARS2 position, in a central region between oriC and the termination site, is important for timely initiation (Kasho et al, 2016 Genes Cells.; 2017 J Biol Chem.).
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| Free Research Field |
分子生物学
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