The regulation of local translation by neuronal RNA binding protein HuD

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18480
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Kindai University
• Principal Investigator: FUKAO Akira 近畿大学, 薬学部, 助教 (50733979)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 翻訳制御 / RNA結合蛋白質

Outline of Final Research Achievements

In neurons, the post-transcriptional regulation of gene expression is essential for the immediate response to external signals. Then, RNA binding protein is a switch for mRNA translation in response to signal. In this study, we analyzed molecular mechanism of maintaining the mRNA translation "OFF" state while the neuron specific RNA binding protein HuD is transported to the appropriate "place" together with the target mRNA ,using the unique biochemical technique of our laboratory. For the known factors (KIF3A, IMP1, SMN) whose interactions with HuD, the effect on translation of mRNA was evaluated using an in vitro translation system. As a result, it was revealed that SMN inhibits the translational activation ability of HuD depending on the binding ability of SMN with HuD.

Free Research Field

分子生物学