2015 Fiscal Year Research-status Report
New Data-Driven Tools to Quantify Heterogeneous Microenvironments in Live Cell Images
Project/Area Number |
15K18511
|
Research Institution | Hokkaido University |
Principal Investigator |
Taylor Nicholas 北海道大学, 電子科学研究所, 特任助教 (50750824)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | Raman Microscopy / Live-Cell Imaging / Data Science / Machine Learning |
Outline of Annual Research Achievements |
Raman microscopy is being used to image living cells, and has the capability to capture details of cellular processes without the need for intrusive labeling of compounds within the cell. Raman microscopic images of living cells contain rich information about the cell, however identification of cellular components is hindered by optical background contamination. An objective, data-driven method was developed to quantify and remove background contamination. A method to discriminate cellular components based on their Raman scattering spectra was successfully developed. Furthermore it was also found that the quantification of background contamination aids in the discrimination process by allowing for the exclusion of spectra containing only background from the discrimination process.
|
Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The first goal of the proposed research plan, quantifying background contamination, has been achieved. A manuscript for publication is in preparation. In addition, the achievement of this research goal provided an unexpected benefit to another research goal, identifying cellular components with data clustering methods. The achievement of this goal is well underway.
|
Strategy for Future Research Activity |
Once the identification and discrimination of cellular components is shown to be reliable, the research will progress towards identifying relationships among time-series of Raman microscopic images. In this line of research, previously achieved research goals will work together towards the greater goal of identifying and recognizing patterns in important cellular processes such as cell division and cell death.
|
Causes of Carryover |
Travel visits to research collaborators at Osaka University. Travel to conferences to share and promote research. Publication of research in peer-reviewed scientific journals.
|
Expenditure Plan for Carryover Budget |
500,000 円 for visits to collaborators. 500,000 円 for travel to research conferences to present research. 700,000 円 for publication fees.
|
Research Products
(2 results)