2017 Fiscal Year Final Research Report
Understanding the mechanism of structural stability for thermophilic rhodopsin
| Project/Area Number |
15K18519
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hokkaido University (2017)
Okayama University (2015-2016) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ロドプシン / 膜タンパク質 / イオンポンプ / 構造安定性 / 高度好熱菌 |
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| Outline of Final Research Achievements |
This research was carried out for a photoreceptor protein called thermophilic rhodopsin (abbreviated as TR), which we have discovered from a thermophilic bacterium for the first time in 2013, (1) to reveal the structural factor for the high thermal stability of TR, and (2) to evaluate the structural stability against some physical and chemical stimuli other than heat. For the purpose (1), it was revealed that TR was highly stabilized by hydrophobic interactions inside the protein by combining the analyses of UV-Vis absorption spectra, X-ray crystallography, and molecular dynamics simulation. For the purpose (2), the exhausted evaluation for the structural stability by using some chemical reagents revealed the correlation between the mechanism for stabilizing the TR structure against heat and that against some chemical stimuli.
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| Free Research Field |
生物物理学
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