2017 Fiscal Year Final Research Report
Selective inheritance of mitochondria from mother cells into spores in fission yeast
| Project/Area Number |
15K18525
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ミトコンドリア / 分裂酵母 / 配偶子形成 / エネルギー代謝 / ATP / オーロラキナーゼ / バイオセンサー |
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| Outline of Final Research Achievements |
We have shown that a meiosis-specific protein Npg1 is involved in efficient sporulation and spore viability in fission yeast. To further elucidate its molecular mechanism, in this study, we first explored upstream factor that regulates intracellular localization and function of Npg1. Our results suggested that aurora-B kinase Ark1 may regulate localization and function of Npg1 after meiosis I and during sporulation.
We also examined intracellular energy status during meiosis and sporulation by visualizing intracellular ATP levels using an ATP biosensor QUEEN. As meiosis and sporulation progressed, ATP levels in mother cells (zygotes or asci) gradually decreased, while ATP levels in prespores and spores increased. These results suggest a well-controlled inheritance of energy metabolism during spore assembly, which we hypothesized in the application of this study. Now we are trying to elucidate mechanisms that regulate cellular energy status during sporulation at a molecular level.
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| Free Research Field |
細胞生物学
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