Analysis of mechanism of resetting replicative life span by imaging aging-associated factors

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18531
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: Okada Satoshi 九州大学, 医学研究院, 助教 (30734488)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 出芽酵母 / 細胞老化 / オルガネラ / イメージング / 胞子形成

Outline of Final Research Achievements

Budding yeast strains in which ageing-related organelles are visualized by C-terminal tagging with green or red fluorescent protein were constructed. Live cell imaging conditions for these strains were optimized. To achieve long-term time-lapse live cell imaging, a microfluidics-based stage top cell culture device was introduced on a microscope. On the device, cell culture conditions for high efficiency sporulation induction were optimized. By development of BiFC system of novel bright fluorescent protein, RENT complexes containing an ageing-related factor, Sir2, were successfully visualized in living yeast cells.

Free Research Field

細胞生物学