Analyses of the circadian clock entrainment and cell-cell phase synchronization mechanisms using a device of local LED light irradiation to the micro-area in higher plants

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18554
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Ito Shogo 京都大学, 理学研究科, 助教 (60632586)
• Research Collaborator: Oyama Tokitaka 京都大学, 理学研究科, 准教授 (30324396)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 概日時計 / ウキクサ / 光受容体 / 局所光 / 形質転換 / ゲノム編集

Outline of Final Research Achievements

The circadian clock system is basically cell-autonomous in plants. On the other hand, circadian clock phase of individual cells are presumably influenced by other cells.
In order to understand the mechanisms of intercellular clock phase communication among cellular circadian clocks in plants, we have utilized a single-cell bioluminescence monitoring system in higher plants. In this project we newly developed a manipulation system of illumination on a microarea in duckweed plants for long-term experiments of circadian rhythms. We combined this device with the bioluminescence reporter imaging technology. During this project we also established a stable transformation methods in a duckweed lemna minor and lemna turionifera. This tecunique also enable us to analyse circadian phase synchronization mechanisms between tissues and/or individual organs.

Free Research Field

光合成生物の時間生物学、基礎生物学、植物分子・生理科学