2016 Fiscal Year Research-status Report
Role of a novel DNA demethylation enzyme in cellular memory
| Project/Area Number |
15K18558
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ブザス ディアナ・ミハエラ 筑波大学, 生命環境系, 准教授 (00616229)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | methylation |
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| Outline of Annual Research Achievements |
Computational analysis of DNA methylone in mutants of the FeS biogenesis pathway that localise to mitochondria revealed some interesting features about putative nuclear processes under control from mitochondria.
Mutants in new candidates from Fe-S biogenesis pathway that emerged from yeast studies are constructed to test their involvement in DNA demethyltion by DRE2.
Genome editing is being used to engineer DRE versions that may alter specifically part of the protein function.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The transition to independent laboratory is a lot slower than expected. In addition, there are inherent technical issues associated with initial set up for experimental molecular biology.
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| Strategy for Future Research Activity |
Transgenics with modified, attenuated and inducible versions of the DRE2
protein in different cellular compartments will be created and profiled
for de-regulated gene sets, leading to elucidation of what biological
roles are connected to the nucleus-mithocondria communication pathway
disturbed in dre2-/-.
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| Causes of Carryover |
Fine adjustment was difficult to make
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| Expenditure Plan for Carryover Budget |
Amount will contribute to research expenses in last year of the grant
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