2016 Fiscal Year Final Research Report
Neuromodulatory funstion of microglia in neural circuits
| Project/Area Number |
15K18575
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | ミクログリア |
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| Outline of Final Research Achievements |
I first analyzed the basic physiological properties of microglia in vitro by combining electrophysiology, calcium imaging, and membrane potential imaging. Specifically, we found that voltage-gated proton channel, one of voltage-gated ion channels of microglia, is important for the regulation of reactive oxigen species (ROS) production. It also contributed to infarct volume in brain ischemia in age-dependent manner.
Next, in order to analyze the importance of this phenomenon in vivo, we started the experiment using zebrafish, which is an excellent animal modle for in vivo study. I used CRISPR/Cas9 system and succeeded in establishing Voltage-gated proton channel deficient zebrafsih. Furthermore, we succeeded in establishing the ROS measurement system in zebrafish, allowing us to perform further detailed analysis in near future.
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| Free Research Field |
生理学
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