How do evolutionary novelties emerge? An approach with genome editing

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18586
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Kyoto University
• Principal Investigator: Koshikawa Shigeyuki 京都大学, 白眉センター, 特定助教 (30714498)
• Research Collaborator: FUKUTOMI Yuichi 京都大学, 大学院理学研究科, 大学院生 ; Matsumoto KEIJI 京都大学, 大学院理学研究科, 研究員
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 進化発生学 / ショウジョウバエ / 模様 / ゲノム編集

Outline of Final Research Achievements

We attempted to introduce genome editing technique by CRISPR / Cas9 system to Drosophila guttifera, aiming to clarify the role of genes in color pattern formation and the regulatory relationship among genes.
We first established a method to quantitatively measure development of pigmentation in wings by image analysis. Next, with reference to the method used in Drosophila melanogaster CRISPR / Cas 9 system, genes on the X chromosome were targeted to show the effectiveness of the method. As a result of trial and error, we established a method to make G0 mosaic individuals and knockout strains with mutation in the germline.

Free Research Field

進化発生生物学