2016 Fiscal Year Final Research Report
Evolution of dimorphic immunoproteasome subunit gene
| Project/Area Number |
15K18587
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | プロテアソーム / プロテアーゼ / 二型性 / 種を超えた多型 |
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| Outline of Final Research Achievements |
PSMB8 is one of the proteasome beta subunits with protease activity responsible for generating MHC class I presenting-peptides. PSMB8 gene of cartilaginous and bony fish has two (A and F) lineages showing about 20% differences of amino acid residues. In bony fish, two lineages are presented as dimorphic alleles maintaining for about 400 million years among species. To clarify the biological factors maintaining the dimorphic alleles, cleavage specificities were compared between A and F alleles. Whereas immunoproteasome containing A lineage of PSMB8 increased chymotrypsin-like activity compared with constitutive proteasome, it having F lineage of PSMB8 decreased. this result implied that two lineages of PSMB8 supply the MHC class I presenting-pepties with various C-terminal residues.
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| Free Research Field |
免疫進化生物学
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