2016 Fiscal Year Final Research Report
Analysis of the novel mode of action of fungicide "protein prenylation" that has been indicated by whole genome analysis
| Project/Area Number |
15K18655
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | The University of Shiga Prefecture |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 殺菌剤 / プレニル化 / ゲラニルゲラニルトランスフェラーゼ |
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| Outline of Final Research Achievements |
In our previous study, it was suggested that tolnifanide resistance gene encodes geranylgeranyl transferase (GGT1). In this study, we introduced the mutated GGT1 gene (C406Y) into the wild-type strain of Bipolaris maydis. The transformants expressing mutated GGT1 showed highly resistance to tolnifanide. We attempted gene knockout of GGT1 but no gene-disrupted strain was obtained, suggesting that GGT1 is essential gene in B. maydis. As a result of examining the localization of RHO1, predicted to undergo geranylgeranylation modification, it was suggested that tolnifanide may inhibit localization of RHO1.
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| Free Research Field |
菌類分子遺伝学、植物病理学、農薬科学
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