Elucidation of molecular mechanisms involved in bacterial biofilm inhibition

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18670
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: OGASAWARA Hiroshi 信州大学, 学術研究院総合人間科学系, 助教 (30535232)
• Research Collaborator: ISHIHAMA Akira 法政大学, マイクロナノテクノロジー研究センター, 客員教授 (80019869) ; KATO Yuki 信州大学, 大学院・応用生物科学科, 大学院生 ; ISHIZUKA Toshiyuki 信州大学, 大学院・応用生物科学科, 大学院生 ; SANO Kotaro 信州大学, 大学院・応用生物科学科, 大学院生 ; MASHUI Shohei 信州大学, 大学院・応用生物科学科, 大学院生 ; HOTTA Shuhei 信州大学, 繊維学部, 大学生 ; KOBAYASHI Hiroaki 信州大学, 繊維学部, 大学生 ; AOKI Michiko 信州大学, 繊維学部, 大学生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Biofilm formation / CsgA / CsgD / Escherichia coli / Amyloid fimbriae / Biofilm inhibition / Transcription factor / Periplasm

Outline of Final Research Achievements

In biofilm formation of Escherichia coli, amyloid fimbriae and cellulose are the major component involved in cell assembly and surface attachment. In this study, we clarified that the expression of E. coli biofilm master regulator CsgD, and the amyloid fimbriae subunits CsgA are negatively regulated by YehT/YehU two component regulatory system. In addition, comprehensive analysis using the collection of the purified transcription factors (TFs) and a set of plasmid collection for overproduction of TFs, we identified some species of TFs that specifically bind to the csgBA promoter and regulates its expression. We also found the novel periplasmic protein YccT which regulated by CsgD involved in the inhibition of amyloid fiber composed of CsgA, and we also clarified the roles involved in the inhibition of csgBA expression. Based on the results of this research, we aim to elucidate the molecular mechanism of inhibition of bacterial biofilm formation.

Free Research Field

分子生物学、ゲノム微生物学