Understanding of oxidative fermentation for next-generation fermentation technology: Regulation of intracellular metabolism following oxidative fermentation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18673
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Ehime University
• Principal Investigator: Ano Yoshitaka 愛媛大学, 農学研究科, 准教授 (00403642)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 酢酸菌 / 酸化発酵 / 発酵生産 / 代謝制御

Outline of Final Research Achievements

Oxidative fermentation is a rapid and effective microbial conversion and is expected as next-generation fermentation technology. In this study, we focused on intracellular assimilation pathway which progresses after extracellular oxidation in acetic acid bacteria to clarify its regulation system.
This study provided the following results using 5-ketogluconate (5KGA) fermentation as a model fermentation. (1) We found transporter involved in 5KGA uptake. (2) Activation of intracellular 5KGA metabolism was more important than induction of 5KGA transporter for induction of 5KG assimilation. (3) We found transcriptional regulator related on 5KGA assimilation. These data will lead to improvement of industrial production of 5KGA.

Free Research Field

応用微生物学