Enzymatic synthesis of useful hydroxyproline derivatives by regio- and stereo-selective hydroxylation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18677
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Waseda University
• Principal Investigator: HARA Ryotaro 早稲田大学, 理工学術院総合研究所(理工学研究所), 次席研究員(研究院講師) (70553535)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: プロリン / ヒドロキシプロリン / エクトイン / 水酸化酵素 / 微生物変換

Outline of Final Research Achievements

The objective of this study was to develop an enzymatic production process for trans-3-hydroxyproline. Sequence-based screening for a novel proline hydroxylating enzyme approach allowed the identification of a proline trans-3-selective hydroxylation activity in ectoine hydroxylase from Streptomyces cattleya. Using the Escherichia coli expressing the ectoine hydroxylase gene as a biocatalyst, an efficient trans-3-hydroxyproline production was achieved. Further, a novel trans-3-hydroxyproline synthetic route was developed via arginine hydroxylation, hydrolysis, and cyclodeamination in this order.

Free Research Field

農学