2017 Fiscal Year Final Research Report
Modulation of organelle localized ion transporter by lipid molecule.
Project/Area Number |
15K18678
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Tohoku University |
Principal Investigator |
Hamamoto Shin 東北大学, 工学研究科, 助教 (10533812)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | イオンチャネル / 出芽酵母 / 液胞 / カルシウム |
Outline of Final Research Achievements |
TRPY1 is a major cation channel localized in yeast vacuole membrane. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the TRPY1 activity. We have identified the Cys crucial for the functional property of the TRPY1 channel in the presence of reducing agent. Next we examined the effect of phosphatidyl inositol phosphate on TRPY1 function since there are several reports on the involvement of PI(3,5)P2 in hyperosmotic stress induced Ca2+ release from organelle. We assessed whether other phosphatidylinositol phosphates (PIPs) affect to the TRPY1 activity, which is monitored as aequorin-mediated luminescence in yeast mutants defective in phosphorylation and dephosphorylation of PIPs. One of mutant of PIP phosphatase showed decreased activity in hyperosmotic stress induced Ca2+ release. Furthermore, the patch-clamp analysis revealed that addition of PI(3)P to the cytoplasmic side reduced the whole-vacuole currents of TRPY1.
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Free Research Field |
生化学、電気生理学
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