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2016 Fiscal Year Final Research Report

Structural and functional analysis of acyltransferase that possesses a unique substrate specificity

Research Project

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Project/Area Number 15K18679
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionTokyo Institute of Technology

Principal Investigator

Miyanaga Akimasa  東京工業大学, 理学院, 助教 (10623126)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywords微生物酵素 / 結晶構造解析 / ポリケタイド合成酵素 / アシル基転移酵素 / タンパク質間相互作用 / クロスリンク
Outline of Final Research Achievements

Acyltransferases are key determinants of building block specificity in polyketide biosynthesis. Despite the importance of protein-protein interactions between acyltransferase and acyl carrier protein (ACP) during the acyltransfer reaction, the mechanism of ACP recognition by acyltransferase had not been understood in detail. An acyltransferase VinK transfers a dipeptide group between two ACPs, VinL and VinP1LdACP, in vicenistatin biosynthesis. In this study, we attempted to crystallize the VinK-ACP complexes. Because transient enzyme-ACP complexes are difficult to crystallize, we developed a covalent cross-linking strategy to trap the VinK-ACP complexes, allowing the determination of the crystal structure of the VinK-VinL complex. The VinK-VinL complex structure allows the first visualization of the interaction between acyltransferase and ACP and provides detailed mechanistic insights into ACP recognition by acyltransferase.

Free Research Field

農学

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Published: 2018-03-22  

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