2017 Fiscal Year Final Research Report
Algal protein kinase TAR1 modulates cellular viability and gametogenesis in carbon/nitrogen imbalance conditions
| Project/Area Number |
15K18682
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 植物生理学 / 植物分子生物学 / 脂質代謝 / 光合成 / 藻類育種 / タンパク質リン酸化 |
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| Outline of Final Research Achievements |
When nutrient deprived, microalgae accumulate triacylglycerol (TAG) in lipid droplets. Dual-specificity kinase, TAG-accumulation-regulator-1 (TAR1) has been reported to regulate carbon metabolism in a green alga, Chlamydomonas reinhardtii. TAR1 is required for acetate-dependent TAG accumulation and degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions. We report that the tar1-1 mutant maintained high levels of cell viability and lower generation of H2O2 compared with wild-type (WT) cells in photoautotrophic N-deficient conditions. Mating efficiency and mRNA abundance of key regulators in gametogenesis were lower in tar1-1 than those in WT. In addition, tar1-1 accumulated higher levels of TAG and starch per cell volumes compared with WT. We identified total 426 TAR1-dependent phosphoproteins by phosphoproteomic analysis. Several protein kinases and enzymes related to N assimilation and carbon metabolism were included.
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| Free Research Field |
植物分子生物学
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