Structural basis of the self- and non-self-recognition in plant self-incompatibility

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18683
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Murase Kohji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50467693)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 植物

Outline of Final Research Achievements

Many flowering plants have self-incompatibility for promotion of out-crossing to maintain the genomic diversity. In Brassica, pistil factor SRK and pollen factor SP11 coding in the S-locus control the self-incompatibility reaction. This pollen rejection is triggered by S-haplotype specific SRK-SP11 interaction. To study the mechanism of S-haplotype specific recognition of SP11 by SRK, we tried to structure determination of SRK-SP11 complex by X-ray crystallography. Here, he successfully expressed the recombinant SRK protein in insect cells, and obtain the large amount of purified SRK protein. We also obtained the protein crystals of SRK-SP11 complex. Our data will contribute to the determination of SRK-SP11 structure and understanding the mechanism of self-incompatibility in plants.

Free Research Field

農芸化学