Production of vitamin D hydroxylated compounds

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18686
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Toyama Prefectural University
• Principal Investigator: Yasuda kaori 富山県立大学, 工学部, 助教 (70707231)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ビタミンD水酸化体 / シトクロムP450 / 骨粗鬆症

Outline of Final Research Achievements

There are lots of vitamin D hydroxylated compounds, and some of them are useful as medical drugs or authentic standard. In this study, we aimed to provide some vitamin D hydroxylated compounds such as active form of vitamin D2 by bioconversion process. We have reported the production of active form of vitamin D3 using the actinomycete CYP105A1 double variant, R73A/R84A. In this study, we found the new triple variant R73A/R84A/M239A to produce active form of vitamin D2. We also clarified that R73A/R84A was useful for the production of 25,26-dihydroxyvitamin D2, which is one of the vitamin D2 metabolite in the human body.

Free Research Field

農学・農芸化学・応用生物化学