Improvement of oocyte competence by enhancing EGFR signaling in cattle

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K18768
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Sugimura Satoshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (00728454)
• Research Collaborator: Gilchrist Robert B ニューサウスウェルズ大学, 特任准教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: EGFR / cAMP / 卵子分泌因子 / 卵丘細胞卵子複合体 / ウシ

Outline of Final Research Achievements

Enhancement of EGF receptor (EGFR) signaling is milestone for acquisition of oocyte developmental competence. However, there are limited information in the mechanism. In preset study, we observed that FSH administration prior to ovum pick up improved oocyte competence. RNA-seq showed that cumulus cells receiving FSH-priming were enhanced EGFR signaling. To mimic the FSH administration in vitro, cumulus-oocyte compelexs (COCs) were tread with cAMP modulator, also known as the second messengers of FSH, before in vitro maturation (pre-IVM). The pre-IVM improved oocyte comptence and ehnaced EGFR signaling. Hence, cAMP may enhance EGFR signaling in bovine COCs.

Free Research Field

動物生殖学