2017 Fiscal Year Final Research Report
Diagnostic possibility of urinary exosomal RNAs for drug-induced kidney injury in rats
| Project/Area Number |
15K18784
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 尿中exosome RNAs / バイオマーカー / 薬剤性腎障害 |
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| Outline of Final Research Achievements |
In this study, I examined diagnostic possibility of urinary exosomal RNAs for drug-induced kidney injury. The combination method of differential centrifugation and spin column was relatively better to extract urinary exosomal RNAs than the commercial kit to extract exosomal RNA. The kidney function in drug-induced nephrotic syndrome model rats was the worst at 5 days after drug injection (day 5). The urinary exosomal RNAs samples on day 5 were extracted and miRNA PCR array was performed. The detected 155 miRNAs were analyzed by pathway analysis software, and inflammatory network was visualized. In conclusion, it is possible that urinary exosomal miRNAs related to inflammation are the candidates of diagnostic biomarker for nephrotic syndrome.
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| Free Research Field |
獣医薬理学
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