2017 Fiscal Year Final Research Report
Detection of a soluble form of CTLA-4 in dog and cat, and application to the diagnostic marker for autoimmune disease.
| Project/Area Number |
15K18788
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 可溶性CTLA-4 / 自己免疫疾患 / 犬 / 診断マーカー |
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| Outline of Final Research Achievements |
For the detection of a soluble form of CTLA-4 (sCTLA-4) in dog, RT-PCR using cDNA obtained from canine PBMC was performed. As a result, alternatively spliced mRNA lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene was detected. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
Next, ELISA kit was used to assess the expression of serum sCTLA-4 in dog with autoimmune disease. However, there was no significant difference in the expression level of serum sCTLA-4 between autoimmune disease patients and healthy control.
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| Free Research Field |
獣医内科学
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