2016 Fiscal Year Final Research Report
Genome-wide analysis of DNA methylation in canine lymphoma
| Project/Area Number |
15K18789
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | DNAメチル化 / エピジェネティクス / リンパ腫 |
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| Outline of Final Research Achievements |
Next-generation sequencing for 5 canine lymphoma cell lines was found to be quantitative so that 100,000 CpG sites could be analyzed. It also showed clearly difference in DNA methylation patterns between lymphoma cell lines and normal lymphocytes. We identified 1000-4000 hypermethylated and 3000-10000 hypomethylated CpG sites in each cell line. Genes known to be involved in tumor were found to be located close to the CpG sites hypermethylated in the cell lines.
The analysis for 24 spontaneous lymphoma cases indicated that DNA methylation pattern could be prognostic factor in canine lymphoma.
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| Free Research Field |
分子生物学
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