2015 Fiscal Year Research-status Report
Ecological function of wood insect associated microbes
| Project/Area Number |
15K18812
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| Research Institution | Hokkaido University |
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Principal Investigator |
高須賀 太一 北海道大学, (連合)農学研究科(研究院), 助教 (70748409)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Wood wasp / cellulolytic microbes / proteomics / biochemistry |
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| Outline of Annual Research Achievements |
In this study, we attempted to identify the microbes symbiont in the wood wasp from Hokkaido Forest. We were able to capture two wood wasp species, Sirex nitobei, and Xiphydria camelus. One bacteria and one fungus were isolated from a mycangia of X. camelus, and were determined to be Rahnella spp., and Daldinia decipiens species by 16S and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose and wood biomass as sole carbon source.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Capturing the woodwasps from native wood is not trivial task. We are hoping to collect more woodwasp insects in this year (2016) in order to increase sample numbers and number of microbes from wood devastating insects to determine their cellulolytic capability.
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| Strategy for Future Research Activity |
The Biomass-degrading potential in the secretome from D. dicipiens was determined. We will be identifying the enzymes that degrade biomass by secretome analysis.
In order to statistically analyze the wood wasp symbionts, we will capture more wood wasp insects from the Hokkaido forest.
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| Causes of Carryover |
We captured wood devastating insects less than what we expected, and the next generation sequencing analyses were not carried out. Thus, we needed to carry over the funding to the next fiscal year for upcoming insect-microbe analyses.
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| Expenditure Plan for Carryover Budget |
I will hire "学術研究員" to support this project.
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