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2016 Fiscal Year Annual Research Report

Ecological function of wood insect associated microbes

Research Project

Project/Area Number 15K18812
Research InstitutionHokkaido University

Principal Investigator

高須賀 太一  北海道大学, 農学研究院, 助教 (70748409)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywordsinsect-microbe symbiont
Outline of Annual Research Achievements

In this study, we attempted to identify the woodwasp symbiont microbes from Hokkaido forest. We captured two wood wasp species, Sirex nitobei and Xiphydria cannelus. One bacteria and one fungus were isolated from a mygcangia of X. canelus, and were identified as Rahnella spp., and Daldinia decipiens species by 16s rRNA and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose, wood biomass as sole carbon source, and glucose was used as a control culture. By utilizing proteomics, we were able to determined multiple glycoside hydrolase family enzymes, which are predicted to break down various polysaccharides present in woody biomass.

  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] Characterization of biomass degradation by fungal and bacterial enzymes from wood devastating insect symbionts2016

    • Author(s)
      HORI Chiaki, SONG Ruopu, TOBIMATSU Yuki, MINKOFF Benjamin, TAKASUKA Taichi
    • Organizer
      日本生化学会年会
    • Place of Presentation
      仙台国際センター(宮城県仙台)
    • Year and Date
      2016-09-25 – 2016-09-27

URL: 

Published: 2018-01-16  

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